Biohazard teaches the players the basics of scientific inquiry (observing, generating hypotheses, gathering data, analyzing findings, developing conclusions, refining hypotheses) while allowing them to explore their "practical" application in a simulated environment. Players develop a comprehensive understanding of the anatomical and physiological systems of the human body. As a medical practitioner, the player can use instruments and resources available to a fully qualified medical practitioner. Through the character, the player uses these tools to attain a deeper understanding of how multiple body systems and organs interact and function.

Specific Advanced Placement Biology areas, covered in Biohazard include:

- Molecules and Cells
- Chemistry for Life
- Water
- Organic Molecules in Organisms
- Free Energy Changes
- Enzymes
- Membranes
- Subcellular Organization
- Cell Cycle and Cell Regulation
- Cellular Energetics
- Fermentation and Cellular Respiration
- Heredity
- Meiosis and Gametogenesis
- Inheritance Patterns
- Molecular Genetics
- Mutation
- Viral Structure and Replication
- Reproduction, Growth and Development
- Structural, Physiological, and Behavioral Adaptations
- Response to the Environment
- Ecology

In addition, players learn when and how to use the following medical tools to aid in their investigation:

- Thermometer
- Stethoscope
- Electron microscope
- Spinal tap/CSF (cerebrospinal fluid)
- History notes
- Blood test (immune response and/or sepsis/differential cell counts)
- X-ray
- Blood pressure
- Blood gases and biochemistry (urea and electrolytes)

Copyright, 2002, MIT.